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differentiation medium dm-2-l1 (ZenBio)

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ZenBio differentiation medium dm-2-l1
Differentiation Medium Dm 2 L1, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/differentiation+medium+dm-2-l1/differentiation+medium+dm2+l1/pm40460832-378-10-13
Average 90 stars, based on 1 article reviews

differentiation medium dm-2-l1 - by Bioz Stars, 2026-09

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Article Title: Hkat, a novel nutritionally regulated transmembrane protein in adipose tissues
Article Snippet: To differentiate 3T3-L1 cells into adipocytes, we used preadipocyte medium (Catalog number PM-1-L1, Zenbio, Research Triangle Park, NC), differentiation medium (DM-2-L1) and adipocyte maintenance medium (AM-1-L1).

Article Title: Adipocytes derived from PA6 cells reliably promote the differentiation of dopaminergic neurons from human embryonic stem cells.
Article Snippet: The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons.. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA).. The level of SDIA has been found to vary considerably between and within batches of PA6 cells.

Article Title: Visceral fat lipolysis by pancreatic lipases worsens heart failure.
Article Snippet: After that, cells were differentiated for three days in a differentiation medium (DM-2-L1, ZenBio, Inc., USA).

Article Title: Glyceollins, soy isoflavone phytoalexins, improve oral glucose disposal by stimulating glucose uptake.
Article Snippet: Soy glyceollins, induced during stress, have been shown to inhibit cancer cell growth in vitro and in vivo.. In the present study, we used prediabetic rats to examine the glyceollins effect on blood glucose.. During an oral glucose tolerance test (OGTT), the blood glucose excursion was significantly decreased in the rats treated with oral administration of either 30 or 90 mg/kg glyceollins.

Cell Culture:

Article Title: Investigation on the Biological Safety and Activity of a Gluconolactone-Based Lotion for Dermocosmetic Application
Article Snippet: PCi-SEB_CAU were grown in PhenoCULT-SEB sebocytes basal culture medium with supplement A (Phenocell, Grasse, France). .. Furthermore, 3T3-L1 pre-adipocytes were cultured in 3T3-L1 pre-adipocytes medium (Zen-Bio, Durham, NC, USA); two days after reaching confluence, the culture medium was switched to differentiation medium (DM-2-L1, Zen-Bio, Durham, NC, USA) for 3 days at 37 °C. .. Then, cells were maintained in maintenance medium (AM-1-L1, Zen-Bio, Durham, NC, USA) that was replaced every 2 days.

Article Title: Mechanisms of Adipocytokine-Mediated Trastuzumab Resistance in HER2-Positive Breast Cancer Cell Lines
Article Snippet: .. During days 6 to 9, cells were cultured in Differentiation Medium (DM-2-L1; Zen-Bio), which contains isobutylmethylxanthine and a PPARy agonist. ..

Microscopy:

Article Title: Targeting endogenous fatty acid synthesis stimulates the migration of ovarian cancer cells to adipocytes and promotes the transport of fatty acids from adipocytes to cancer cells
Article Snippet: Murine 3T3L1 fibroblasts (Zen-Bio Inc.) at 5×10 3 /cm 2 were cultured for 7 days in 'pre-adipocyte medium' with the addition of 1% penicillin/streptomycin/amphotericin (PSF; Zen-Bio Inc), changing the medium each day until the cells reached 100% confluency. .. After 2 days, the medium was changed to 'differentiation medium' (Zen-Bio Inc., cat. no. DM-2-L1) with the addition of PSF and the cells were allowed to differentiate into adipocytes for 72 h. Differentiated cultures were then maintained for 7-14 days in 'adipocyte medium' (Zen-Bio Inc.) with the addition of PSF; the medium was changed every other day and the cells were prepared for further experimentation as indicated by the accumulation of large lipid droplets in the cells using phase contrast microscopy with an Olympus IX73 microscope (Olympus Corporation) ( ). ..

Article Title: Targeting endogenous fatty acid synthesis stimulates the migration of ovarian cancer cells to adipocytes and promotes the transport of fatty acids from adipocytes to cancer cells.
Article Snippet: Murine 3T3L1 fibroblasts (Zen‐Bio Inc.) at 5x103/cm2 were cultured for 7 days in ‘pre‐adipocyte medium’ with the addition of 1% penicillin/streptomycin/amphotericin (PSF; Zen‐Bio Inc), changing the medium each day until the cells reached 100% confluency. .. After 2 days, the medium was changed to ‘differentiation medium’ (Zen‐Bio Inc., cat. no. DM‐2‐L1) with the addition of PSF and the cells were allowed to differentiate into adipocytes for 72 h. Differentiated cultures were then maintained for 7‐14 days in ‘adipocyte medium’ (Zen‐Bio Inc.) with the addition of PSF; the medium was changed every other day and the cells were prepared for further experimentation as indicated by the accumulation of large lipid droplets in the cells using phase contrast microscopy with an Olympus IX73 microscope (Olympus Corporation) (Fig. S1A). ..

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T. cruzi infection induces adipocyte apoptosis, releasing adipomes that regulate adipogenic signaling (A) Immunoblot analysis of cleaved Caspase 7 expression in the lysates of 3T3-L1 adipocytes (uninfected, TNFα-treated, and T. cruzi infected) ( n = 3/group). Bar graph values were derived from densitometry analysis and by normalizing target protein expression to β-Actin. (B) Fold change expression of CASP3 , RIPK3, and MLKL mRNA transcripts (normalized to HPRT ) in cultured human adipocytes (uninfected, TNFα-treated and T. cruzi infected) ( n = 3). (C and D) Adipome size distribution and absolute concentration as determined by Spectradyne nCS1 with a C-900 microfluidic cartridge. Both mouse and human adipomes were derived from cultured 3T3-L1 and human adipocyte-conditioned media, respectively. Inset: A (blue) = uninfected, B (green) = TNFα-treated, C (red) = T. cruzi infected. (E) PCR analysis of adipogenic ( Adipoq, Fabp4 , and Pparg ), apoptotic ( Chop ) and extracellular vesicle ( Tsg101 and Cd13 ) marker genes in 3T3-L1-derived adipomes. Lane 1, 2, and 3 represent 3T3-L1-derived adipomes from three independent cultures. Product size: Adipoq , 192bp; Fabp4 , 133bp; Pparg , 132bp; Chop , 118bp; Tsg101 , 103bp; and Cd13 , 121bp. (F and G) Fold change expression of Adipoq mRNA transcripts (normalized to Hprt ) in RAW macrophages (F) and human fibroblasts (G) treated with 3T3-L1- and human adipocyte-derived adipomes, respectively, at 1:1 and 1:50 cell-to-adipome ratio for 48 h ( n = 3). All treatment groups (Treated - A, - B and - C) were compared to untreated groups. The # symbol indicates comparison between Treated - B and - C. Treated - A = adipomes from uninfected adipocytes; Treated - B = adipomes from TNFα-treated adipocytes; and Treated - C = adipomes from T. cruzi -infected adipocytes. Data are represented as mean ± SEM. (∗/# p < 0.05, ∗∗ p ≤ 0.01, ∗∗∗/### p ≤ 0.001 and ∗∗∗∗ p ≤ 0.0001).

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet: T. cruzi infection induces adipocyte apoptosis, releasing adipomes that regulate adipogenic signaling (A) Immunoblot analysis of cleaved Caspase 7 expression in the lysates of 3T3-L1 adipocytes (uninfected, TNFα-treated, and T. cruzi infected) ( n = 3/group). Bar graph values were derived from densitometry analysis and by normalizing target protein expression to β-Actin. (B) Fold change expression of CASP3 , RIPK3, and MLKL mRNA transcripts (normalized to HPRT ) in cultured human adipocytes (uninfected, TNFα-treated and T. cruzi infected) ( n = 3). (C and D) Adipome size distribution and absolute concentration as determined by Spectradyne nCS1 with a C-900 microfluidic cartridge. Both mouse and human adipomes were derived from cultured 3T3-L1 and human adipocyte-conditioned media, respectively. Inset: A (blue) = uninfected, B (green) = TNFα-treated, C (red) = T. cruzi infected. (E) PCR analysis of adipogenic ( Adipoq, Fabp4 , and Pparg ), apoptotic ( Chop ) and extracellular vesicle ( Tsg101 and Cd13 ) marker genes in 3T3-L1-derived adipomes. Lane 1, 2, and 3 represent 3T3-L1-derived adipomes from three independent cultures. Product size: Adipoq , 192bp; Fabp4 , 133bp; Pparg , 132bp; Chop , 118bp; Tsg101 , 103bp; and Cd13 , 121bp. (F and G) Fold change expression of Adipoq mRNA transcripts (normalized to Hprt ) in RAW macrophages (F) and human fibroblasts (G) treated with 3T3-L1- and human adipocyte-derived adipomes, respectively, at 1:1 and 1:50 cell-to-adipome ratio for 48 h ( n = 3). All treatment groups (Treated - A, - B and - C) were compared to untreated groups. The # symbol indicates comparison between Treated - B and - C. Treated - A = adipomes from uninfected adipocytes; Treated - B = adipomes from TNFα-treated adipocytes; and Treated - C = adipomes from T. cruzi -infected adipocytes. Data are represented as mean ± SEM. (∗/# p < 0.05, ∗∗ p ≤ 0.01, ∗∗∗/### p ≤ 0.001 and ∗∗∗∗ p ≤ 0.0001).

Article Snippet: 3T3-L1 Differentiation Medium , ZenBio , Cat#DM-2-L1.

Techniques: Infection, Western Blot, Expressing, Derivative Assay, Cell Culture, Concentration Assay, Marker, Comparison

Adiponectin enables selective capture of adipomes from murine white adipose tissue (WAT) (A) Immunoblot analysis of phospho-Perilipin, cleaved Caspase 7 and Annexin V expression in the WAT lysates of infected (20 DPI) and uninfected C57BL/6J mice (n = 4–8 per group). Bar graph values were derived from densitometry analysis and by normalizing target protein expression to GDI. Error bars indicate the standard error of the mean (∗ p < 0.05). (B) Immunoblot analysis of adiponectin in WAT-derived large-EVs (L-EV) and small-EVs (S-EV). Black arrow points to the 25 kDa marker band on the pre-stained protein ladder. Adiponectin band appears at 26/30 kDa. Lanes: C, control WAT lysate; L Ev, large-EV; and S Ev, small-EV. (C) Surface immunofluorescence staining of 3T3-L1 adipocytes show the co-localization of Adiponectin (green) with Annexin V (red) (top panel) and FABP4 (red) with Annexin V (green) (bottom panel) on budding apoptotic bodies. Scale bar, 50 μm. (D) Immunoblot analysis of adiponectin in WAT-derived large-adipomes and small-adipomes. Black arrow points to the 25 kDa marker band on pre-stained protein ladder. Adiponectin band appears at 26/30 kDa. Lanes: C, control WAT lysate; L, large-adipomes; and S, small-adipomes. (E) Immunofluorescence assay (IFA) of bead-bound adipomes stained with Adiponectin-AF488 (top panel) and FABP4-AF488 (bottom panel) imaged on the FITC channel along with their corresponding bright field (BF) images. Scale bar, 5 μm. (F) WAT-derived adipome size distribution and absolute concentration as determined by Spectradyne nCS1 with a C-2000 microfluidic cartridge. Inset: L (blue), large-adipomes; S (green), small-adipomes. Gate: G1, particle concentration at size range of 250–400 nm diameter showing more small-adipomes than large-adipomes; and G2, particle concentration at size range of 700–1100 nm diameter showing mostly large-adipome distribution. (G) Transmission Electron Microscopy (TEM) images of small-adipomes (left, scale bar 80 nm) and large-adipomes (right, scale bar 100 nm) with a direct magnification of 20,000×. Black arrow indicates the budding scars on L-adipomes.

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet: Adiponectin enables selective capture of adipomes from murine white adipose tissue (WAT) (A) Immunoblot analysis of phospho-Perilipin, cleaved Caspase 7 and Annexin V expression in the WAT lysates of infected (20 DPI) and uninfected C57BL/6J mice (n = 4–8 per group). Bar graph values were derived from densitometry analysis and by normalizing target protein expression to GDI. Error bars indicate the standard error of the mean (∗ p < 0.05). (B) Immunoblot analysis of adiponectin in WAT-derived large-EVs (L-EV) and small-EVs (S-EV). Black arrow points to the 25 kDa marker band on the pre-stained protein ladder. Adiponectin band appears at 26/30 kDa. Lanes: C, control WAT lysate; L Ev, large-EV; and S Ev, small-EV. (C) Surface immunofluorescence staining of 3T3-L1 adipocytes show the co-localization of Adiponectin (green) with Annexin V (red) (top panel) and FABP4 (red) with Annexin V (green) (bottom panel) on budding apoptotic bodies. Scale bar, 50 μm. (D) Immunoblot analysis of adiponectin in WAT-derived large-adipomes and small-adipomes. Black arrow points to the 25 kDa marker band on pre-stained protein ladder. Adiponectin band appears at 26/30 kDa. Lanes: C, control WAT lysate; L, large-adipomes; and S, small-adipomes. (E) Immunofluorescence assay (IFA) of bead-bound adipomes stained with Adiponectin-AF488 (top panel) and FABP4-AF488 (bottom panel) imaged on the FITC channel along with their corresponding bright field (BF) images. Scale bar, 5 μm. (F) WAT-derived adipome size distribution and absolute concentration as determined by Spectradyne nCS1 with a C-2000 microfluidic cartridge. Inset: L (blue), large-adipomes; S (green), small-adipomes. Gate: G1, particle concentration at size range of 250–400 nm diameter showing more small-adipomes than large-adipomes; and G2, particle concentration at size range of 700–1100 nm diameter showing mostly large-adipome distribution. (G) Transmission Electron Microscopy (TEM) images of small-adipomes (left, scale bar 80 nm) and large-adipomes (right, scale bar 100 nm) with a direct magnification of 20,000×. Black arrow indicates the budding scars on L-adipomes.

Article Snippet: 3T3-L1 Differentiation Medium , ZenBio , Cat#DM-2-L1.

Techniques: Western Blot, Expressing, Infection, Derivative Assay, Marker, Staining, Control, Immunofluorescence, Concentration Assay, Transmission Assay, Electron Microscopy

Journal: iScience

Article Title: Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation

doi: 10.1016/j.isci.2024.109672

Figure Lengend Snippet:

Article Snippet: 3T3-L1 Differentiation Medium , ZenBio , Cat#DM-2-L1.

Techniques: Infection, Recombinant, Electron Microscopy, SYBR Green Assay, Lysis, Protease Inhibitor, Plasmid Preparation, XF Assay, Isolation, Quantitation Assay, Bicinchoninic Acid Protein Assay, Software

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